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首页> 外文OA文献 >Human homologs of TU transposon sequences: polypurine/polypyrimidine sequence elements that can alter DNA conformation in vitro and in vivo.
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Human homologs of TU transposon sequences: polypurine/polypyrimidine sequence elements that can alter DNA conformation in vitro and in vivo.

机译：TU转座子序列的人类同源物：可在体外和体内改变DNA构象的聚嘌呤/聚嘧啶序列元件。

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We previously have shown that homologs of the outer domain segment of the inverted repeat termini (IVR-OD) of the sea urchin TU transposons are conserved among multiple eucaryotic species, including humans. We report here that two cloned human DNA IVR-OD homologs, Hut2 and Hut17, consist of a series of tandem repeats of the trimer AGG/TCC, forming segments (313 and 221 base pairs in length, respectively) of polypurine/polypyrimidine (pPu/pPy or "Puppy") asymmetry in the two DNA strands; these are punctuated at certain sites with variant trimers, which are different for the two clones. Sequences homologous to the Hut2 pPu/pPy tract exist at multiple sites in the DNA of a wide variety of eucaryotes. Hybridization of human DNA with a Hut2 probe or with a previously described chicken DNA pPu/pPy sequence indicates that pPu/pPy sequences can be grouped into families distinguishable by the extent of their homology with each probe at different hybridization stringencies. Moreover, particular pPu/pPy tracts show species-specific differences in their distribution. Both the Hut2 and Hut17 pPu/pPy tracts are cleaved by S1 nuclease when tested on supercoiled plasmids. Most if not all of the 313-base-pair Hut2 pPu/pPy tract is also sensitive to S1 in its native location in HeLa cell chromatin, indicating that the sequence contains conformational information that can be expressed in vivo. This view is supported by evidence that exogenously derived Hut2 pPu/pPy tracts introduced into mouse L cells and integrated in chromatin can assume an S1-sensitive conformation.
机译：我们以前已经表明，海胆TU转座子的反向重复末端（IVR-OD）的外域片段的同源物在包括人类在内的多个真核物种之间是保守的。我们在这里报告说，两个克隆的人类DNA IVR-OD同源物，Hut2和Hut17，由三聚体AGG / TCC的一系列串联重复序列组成，形成聚嘌呤/聚嘧啶（pPu）的片段（分别长313和221个碱基对） / pPy或“ Puppy”）在两条DNA链中不对称；它们在某些部位带有变体三聚体，这对于两个克隆而言是不同的。与Hut2 pPu / pPy序列同源的序列存在于多种真核生物DNA的多个位点。人DNA与Hut2探针或先前描述的鸡DNA pPu / pPy序列的杂交表明，pPu / pPy序列可分为不同的家族，这些家族可通过它们与每种探针在不同杂交严格性下的同源性程度加以区分。此外，特定的pPu / pPy片段在其分布中显示出特定于物种的差异。在超螺旋质粒上测试时，Hut2和Hut17 pPu / pPy片段均被S1核酸酶切割。大多数（即使不是全部）313个碱基对的Hut2 pPu / pPy道在HeLa细胞染色质中的天然位置也对S1敏感，表明该序列包含可以在体内表达的构象信息。这种观点得到证据的支持，即引入小鼠L细胞并整合在染色质中的外源性Hut2 pPu / pPy片段可以呈现S1敏感构象。

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